Delivery of Plasmid DNA into Mammalian Cell Lines Using pH-Sensitive Liposomes: Comparison with Cationic Liposomes
Identifieur interne : 002E77 ( Main/Exploration ); précédent : 002E76; suivant : 002E78Delivery of Plasmid DNA into Mammalian Cell Lines Using pH-Sensitive Liposomes: Comparison with Cationic Liposomes
Auteurs : Jean-Yves Legendre [États-Unis, France] ; Francis C. Szoka Jr [États-Unis]Source :
- Pharmaceutical Research [ 0724-8741 ] ; 1992-10-01.
English descriptors
- KwdEn :
Abstract
Abstract: We compare the transfection efficiency of plasmid DNA encoding either luciferase or (β-galactosidase encapsulated in pH-sensitive liposomes or non-pH-sensitive liposomes or DNA complexed with cationic liposomes composed of dioleoyloxypropyl-trimethylammonium:dioleoylphosphatidyl-ethanolamine (1:1, w/w) (Lipofectin) and delivered into various mammalian cell lines. Cationic liposomes mediate the highest transient transfection level in all cell-lines examined. pH-sensitive liposomes, composed of cholestryl hemisuccinate and dioleoylphosphatidylethanolamine at a 2:1 molar ratio, mediate gene transfer with efficiencies that are 1 to 30% of that obtained with cationic liposomes, while non-pH-sensitive liposome compositions do not induce any detectable transfection. Cationic liposomes mediate a more rapid uptake of plasmid DNA, to about an eightfold greater level than that obtained with pH-sensitive liposomes. The higher uptake of DNA mediated by Lipofectin accounts for part of its high transfection efficiency. Treatment of cells with chloroquine, ammonium chloride, or monensin decreases (threefold) transfection using pH-sensitive liposomes and either has no effect on or enhances cationic liposome-mediated transfection. Therefore plasma membrane fusion is not the only mechanism available to cationic liposomes; in certain cell lines DNA delivery via endocytosis is a possible parallel pathway and could augment the superior transfection efficiency observed with cationic liposomes.
Url:
DOI: 10.1023/A:1015836829670
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A44
- to stream Istex, to step Curation: 000A44
- to stream Istex, to step Checkpoint: 001C50
- to stream Main, to step Merge: 002F43
- to stream Main, to step Curation: 002E77
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Delivery of Plasmid DNA into Mammalian Cell Lines Using pH-Sensitive Liposomes: Comparison with Cationic Liposomes</title>
<author><name sortKey="Legendre, Jean Yves" sort="Legendre, Jean Yves" uniqKey="Legendre J" first="Jean-Yves" last="Legendre">Jean-Yves Legendre</name>
</author>
<author><name sortKey="Szoka Jr, Francis C" sort="Szoka Jr, Francis C" uniqKey="Szoka Jr F" first="Francis C." last="Szoka Jr">Francis C. Szoka Jr</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:FAFD87A8493D0D7CA57EB2B96B90D33A78AF3C7A</idno>
<date when="1992" year="1992">1992</date>
<idno type="doi">10.1023/A:1015836829670</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-DQNQX6CZ-W/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000A44</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A44</idno>
<idno type="wicri:Area/Istex/Curation">000A44</idno>
<idno type="wicri:Area/Istex/Checkpoint">001C50</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001C50</idno>
<idno type="wicri:doubleKey">0724-8741:1992:Legendre J:delivery:of:plasmid</idno>
<idno type="wicri:Area/Main/Merge">002F43</idno>
<idno type="wicri:Area/Main/Curation">002E77</idno>
<idno type="wicri:Area/Main/Exploration">002E77</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Delivery of Plasmid DNA into Mammalian Cell Lines Using pH-Sensitive Liposomes: Comparison with Cationic Liposomes</title>
<author><name sortKey="Legendre, Jean Yves" sort="Legendre, Jean Yves" uniqKey="Legendre J" first="Jean-Yves" last="Legendre">Jean-Yves Legendre</name>
<affiliation wicri:level="2"><country>États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>School of Pharmacy, University of California, 94143-0446, San Francisco</wicri:cityArea>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Pharmacotechnie et Biopharmacie, Faculté de Pharmacie, Université Paris XI</wicri:regionArea>
<placeName><settlement type="city">Orsay</settlement>
<region type="region" nuts="2">Île-de-France</region>
</placeName>
<orgName type="university">Université Paris-Sud</orgName>
</affiliation>
</author>
<author><name sortKey="Szoka Jr, Francis C" sort="Szoka Jr, Francis C" uniqKey="Szoka Jr F" first="Francis C." last="Szoka Jr">Francis C. Szoka Jr</name>
<affiliation wicri:level="2"><country>États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>School of Pharmacy, University of California, 94143-0446, San Francisco</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Pharmaceutical Research</title>
<title level="j" type="sub">An Official Journal of the American Association of Pharmaceutical Scientists</title>
<title level="j" type="abbrev">Pharm Res</title>
<idno type="ISSN">0724-8741</idno>
<idno type="eISSN">1573-904X</idno>
<imprint><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="1992-10-01">1992-10-01</date>
<biblScope unit="volume">9</biblScope>
<biblScope unit="issue">10</biblScope>
<biblScope unit="page" from="1235">1235</biblScope>
<biblScope unit="page" to="1242">1242</biblScope>
</imprint>
<idno type="ISSN">0724-8741</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0724-8741</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>(β-galactosidase</term>
<term>Lipofectin</term>
<term>cell culture</term>
<term>gene therapy</term>
<term>liposome</term>
<term>luciferase</term>
<term>pH-sensitive</term>
<term>plasmid</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: We compare the transfection efficiency of plasmid DNA encoding either luciferase or (β-galactosidase encapsulated in pH-sensitive liposomes or non-pH-sensitive liposomes or DNA complexed with cationic liposomes composed of dioleoyloxypropyl-trimethylammonium:dioleoylphosphatidyl-ethanolamine (1:1, w/w) (Lipofectin) and delivered into various mammalian cell lines. Cationic liposomes mediate the highest transient transfection level in all cell-lines examined. pH-sensitive liposomes, composed of cholestryl hemisuccinate and dioleoylphosphatidylethanolamine at a 2:1 molar ratio, mediate gene transfer with efficiencies that are 1 to 30% of that obtained with cationic liposomes, while non-pH-sensitive liposome compositions do not induce any detectable transfection. Cationic liposomes mediate a more rapid uptake of plasmid DNA, to about an eightfold greater level than that obtained with pH-sensitive liposomes. The higher uptake of DNA mediated by Lipofectin accounts for part of its high transfection efficiency. Treatment of cells with chloroquine, ammonium chloride, or monensin decreases (threefold) transfection using pH-sensitive liposomes and either has no effect on or enhances cationic liposome-mediated transfection. Therefore plasma membrane fusion is not the only mechanism available to cationic liposomes; in certain cell lines DNA delivery via endocytosis is a possible parallel pathway and could augment the superior transfection efficiency observed with cationic liposomes.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Île-de-France</li>
</region>
<settlement><li>Orsay</li>
</settlement>
<orgName><li>Université Paris-Sud</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Californie"><name sortKey="Legendre, Jean Yves" sort="Legendre, Jean Yves" uniqKey="Legendre J" first="Jean-Yves" last="Legendre">Jean-Yves Legendre</name>
</region>
<name sortKey="Szoka Jr, Francis C" sort="Szoka Jr, Francis C" uniqKey="Szoka Jr F" first="Francis C." last="Szoka Jr">Francis C. Szoka Jr</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Legendre, Jean Yves" sort="Legendre, Jean Yves" uniqKey="Legendre J" first="Jean-Yves" last="Legendre">Jean-Yves Legendre</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E77 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002E77 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:FAFD87A8493D0D7CA57EB2B96B90D33A78AF3C7A |texte= Delivery of Plasmid DNA into Mammalian Cell Lines Using pH-Sensitive Liposomes: Comparison with Cationic Liposomes }}
This area was generated with Dilib version V0.6.33. |